Les champignons endophytes sont des organismes qui vivent à l’intérieur de communautés de champignons endophytes de racines de deux espèces de. L’inoculation du pois chiche avec des champignons endophytes indigènes en serre a manipulation de leurs champignons endophytes par inoculation. champignons endophytes du “Rhynia gwynne-vaughanii” K. et L. etude morphologique et deductions sur leur biologie []. Boullard, B. Lemoigne, Y.

Author: Banris Kabar
Country: Guinea
Language: English (Spanish)
Genre: Life
Published (Last): 17 March 2014
Pages: 36
PDF File Size: 4.16 Mb
ePub File Size: 3.23 Mb
ISBN: 296-1-75927-355-9
Downloads: 49859
Price: Free* [*Free Regsitration Required]
Uploader: Maulmaran

The position of the attachment of the isoprenyl side chain to enddophytes aromatic side of the indole substructure was evident by the long range correlations of CH 2 -8 to both H-4 and H-6 in the COSY spectrum.

What is an endophytic fungus? We evaluated the antagonistic activity of some endophytes against several fungal pathogens and selected candidate endophytes for future introduction into commercial switchgrass chxmpignons for biomass enhancement.

Canadian Forest Service Publications

The following gradient was used MeOH, 0. Exponentially growing cells were harvested, counted and diluted appropriately. First, the percentage of chsmpignons survival was determined and results were compared to those of untreated controls.

The crude organic extract of the fungal strain was proven to be active when tested for cytotoxicity against LY mouse lymphoma cells. Results and discussion 18 The crude ethyl acetate extract of rice cultures of Chaetomium champignnos. Secondary metabolites from endophytic Streptomyces aureofaciens CMUAc and their antifungal activity.

The yellow MTT penetrates into the living cells and in the presence of mitochondrial dehydrogenases, MTT is transformed to a blue formazan complex. The colour intensity is correlated with the number of healthy living cells.


The primary focus of this study was to isolate the endophytes, and provide taxonomic identifications based on comparative analysis of ITS rDNA gene sequences. Via what appears to be their contribution to the host plant, the endophytes may produce a plethora of substances of potential use to modern medicine, agriculture, and pharmaceutical industry.

A total of endophytes isolates were recovered 52 bacteria and 93 fungi from whole plant samples collected at early vegetative, and full reproductive stages. This study reports the chemical investigation and cytotoxic activity of the secondary metabolites produced by the endophytic fungus Chaetomium sp.

Isocochliodinol and neocochliodinol, bis 3-indolyl – benzoquinones from Chaetomium spp. The relationship that they establish with the plant varies from symbiotic to bordering on pathogenic Strobel, a. Ethnopharmacology75 Historically, it is well known for its fungistatic, virustatic and tannin-based anti-microbial properties.

The compound was previously obtained from several Chaetomium sp. Cochliodinol proved to be highly active against the cancer cell line with an EC50 of 7.

Artificial inoculation of young switchgrass seedlings with selected bacterial endophytes hold promise as a method of reinfection switchgrass seedlings. However, in the case of compound 2this proton signal was the one exhibiting the ortho-coupling, proving that the side chain was situated meta with regard to H-4 and thus resided at C The chemistry of cochliodinol, a chqmpignons of Chaetomium spp.

The material was placed on a Petri dish malt agar medium containing an antibiotic to suppress bacterial growth medium composition: Le champignon endophytique Chaetomium sp. Thus, 2 had to represent a symmetrical isomer of cochliodinol 1leaving a positional isomer with the prenyl group attached to C-6 as the most probable alternative. The fungal strain was identified by PCR.

All items in eScholarship McGill are endophtes by copyright with all eendophytes reserved unless otherwise indicated. Thus, it may be concluded that cytotoxic activity was affected by the position of champignonns substituents at the indole rings.


It has been established that perennial grasses harbour different types of endophytic bacteria and fungi.

Mycoflore des aiguilles de Pinus banksiana et Pinus resinosa. I. Champignons endophytes

Taxonomic identification of the fungus. The small amount of ethanol present in the wells did not affect the experiments. Material and methods 2. Bioprospecting for microbial endophytes and their natural endophytrs.

Search results for “champignons endophytes”

They reside inside the tissues of nearly all healthy plants. Furthermore, aromatic proton and carbon resonances had chemical shifts and multiplicities consistent with the presence of a disubstituted indole residue. Received on February 4,accepted on July 2, In the 1 H NMR spectrum, all resonances originate from pairs of chemically equivalent groups due to symmetry in the molecule.

Taking into consideration that despite champignpns from other drug discovery methods, natural products are still providing their fair share of new clinical candidates and chxmpignons Butler, we intend to continue our specific search for bioactive substances with pharmaceutical potential as well as contribute to the question of the ecological function of secondary metabolites produced by endophytic fungi aiming to a better understanding of this interesting group of organisms.

The antibacterial activity of some naturally occurring 2,5-dihydroxy-1,4-benzoquinones. Topical anti-inflammatory activity of Salvia officinalis L.

Evaluation of a tetrazolium-based semiautomated colorimetric assay: