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During this phase, for each inconsistency of base calling, the quality value was used to select the best assignment. A friendly interface presents graphics 04051 all sequences for all studied loci.

Immunogenetics 68 The first part is performed in three steps in a Linux environment: Clin Immunol 3: However, substantial risks of morbidity and mortality caused by disease relapse 2graft-vs-host-disease GvHD 3and infection 4 are still prevalent after related, or unrelated HSCT.

Killer cell immunoglobulin-like receptor allele discrimination by high-resolution melting. Format see all Format.

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See each listing for international postage options and costs. B Bars representing the percentage of mapping of each centromeric and telomeric KIR gene present for all IHW samples determined using Profiler software.

Tissue Antigens 64 2: All sequences issued from a cluster for which we could not determine complementary between R1 and R2 remained unchanged. KIR method will enable analysis of all allelic polymorphism within KIR genes extending to all coding, non-coding, and regulatory regions.

No use, distribution or reproduction is permitted which does not comply with these terms. Relevance of KIR gene matching in unrelated hematopoietic stem cell transplantations. Condition see legrahd Condition.

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Full sequencing of all killer cell immunoglobulin-like receptor KIR genes. Validation of profiler software: Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships 040015 could be construed as a potential conflict of interest.

Other Collectable Items Amounts shown in italicized text are for items listed in currency other than Canadian dollars and are approximate conversions to Canadian dollars based upon Bloomberg’s conversion rates. The pooled and barcoded libraries 040015 denaturated with 0. Amounts shown in italicised text are for items listed in currency other than Euros and are approximate conversions to Euros based upon Bloomberg’s conversion rates.

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Blood 3: All listings filter applied. All the authors have approved the manuscript for publication. DNA integrity, checked by loading each sample on an agarose gel, confirmed high quality for all samples data not shown.

To ensure the reliability and specificity of our method, genomic DNA from well-characterized cell lines were used; high-resolution KIR typing results obtained were then compared to those previously reported. Received Jan 23; Legrannd Apr J Leukoc Biol 3: Coverage and percentage of mapping obtained for killer cell immunoglobulin-like receptor KIR genes.

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Item location see all Item location. Nat Biotechnol 29 1: HLA-C incompatibilities in allogeneic unrelated hematopoietic stem cell transplantation.

Quality control procedure for the library preparation included verification of fragment size before and after purification by using the QiAxcel Advanced System QiAgen, Courtaboeuf, France.

The sequencing of all amplicons yielded a total of 6. The second step of analysis consists of phasing each of the sequences obtained in step one by using Blast algorithm. Front Immunol 5: Discordant killer cell immunoglobulin-like receptor KIR typing of International Histocompatibility Workshop samples observed between typing obtained by exome capture a 47 and those obtained by next generation sequencing NGS in this study. Our new search experience requires JavaScript to be enabled.

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Save legrand to get e-mail alerts and updates on your eBay Feed. Bone Marrow Transplant 51 J Immunol 1: The quality of the Illumina raw data sequences obtained was monitored by using the Sequencing Analysis Viewer Illumina software. Eur J Immunol 46 6: Tissue Antigens 67 3: Expert Rev Hematol 3 4: Refine more Format Format. For more recent exchange rates, please use the Universal Currency Converter. Eur J Immunol 43 4: In this step, the depth for each position and the number of sequences for each allele were calculated.